The accident happened at Coptic Church near Mamboleo junction along Kisumu Kakamega road.
Kisumu Central police station traffic commandant Jane Mbevi confirmed that three died on the spot.
Mbevi says a passenger vehicle from Kakamega to Kisumu lost control and landed into a ditch.
She says several passengers were pulled out of the wreckage with serious injuries and were rushed to Jaramogi Oginga Odinga Teaching and Referral hospital.
Mbevi says the wreckage was towed to Kondele police station as those who perished were taken to the hospital mortuary.
The accident scene is a black spot that has claimed several lives in the recent past.